Successful venipuncture can be a challenge in turtles and tortoises, however hematology and biochemistry results serve as an important part of the minimum database in chelonians just as they do for all veterinary patients. Use this video, or text with still images, to review the equipment needed and sample handling recommendations as well as the potential complications and proper approach to the jugular vein, brachial vein, subcarapacial vessel, and dorsal coccygeal sinus in the chelonian.
Hematology and biochemistry results are an important part of the minimum database for all veterinary patients, including lizards. Proper venipuncture technique is critical for accurate interpretation of laboratory results. Blood samples are most frequently collected from the ventral coccygeal vein and jugular vein in lizards; however, the site selected can depend on a variety of factors including the preferences and experience of the phlebotomist, the volume of blood needed, patient size and temperament, and of course the species involved.
Proper patient handling, blood collection technique and sample handling are all critical for accurate interpretation of hematology and biochemistry in all patients, including snakes. Use the video or text with still images to review equipment needed as well as the potential complications and proper approach to the ventral coccygeal vein and the heart, the two most common venipuncture sites in the snake.
Dr. Jaime Samour presented Part 1 (medical and nursing procedures) of his presentation for the Avian, Wildlife & Exotics club at the University of Tennessee College of Veterinary Medicine, and Part 2 (cosmetic procedures) for Mississippi State University. These distance-learning events were hosted by the Lafeber Company Student Program.
Long-term vascular access is difficult to obtain and maintain in chelonians. Fortunately, central venous catheters provide flexibility and length to avoid catheter dislodgement. Central lines are an effective tool that allow serial blood measurements and can be used for anesthesia administration, intravenous drug delivery, blood product transfusions, and continuous fluid therapy or continuous rate infusions.This photo tutorial article describes this simple technique step-by-step.
Hematology and biochemistry results serve as an important part of the minimum database for all veterinary patients. Although collection of blood samples can be a clinical challenge in reptiles, the method of patient handling, blood collection and sampling techniques are all critical for proper interpretation of laboratory results. This brief video or slideshow with still images reviews the basic principles of reptile venipuncture that should be considered before, during, and after the procedure.
Blood work is considered a basic diagnostic test in every species, including birds. Venipuncture may be indicated for wellness screening, sample collection for DNA sexing, evaluation of the ill or injured bird, as well as collection of blood for transfusion. The value of testing must always be weighed against the stress of venipuncture since the critically ill bird may not be stable enough for restraint. This article reviews equipment needed, the volume of the blood sample, general tips for blood collection, common venipuncture sites in the bird, and sample handling.
Blood collection is challenging in sugar gliders. Heavy sedation or general anesthesia, using isoflurane or sevoflurane, is almost always required in clinical practice. The most common sites for blood collection in the glider are the jugular vein and the…
Blood collection is challenging in rats and mice, and heavy sedation or general anesthesia is almost always required in clinical practice. Increasing patient body temperature to promote vasodilation can also be helpful. Gently warm the rodent by placing its cage on a heating pad set on low or by placing the cage in an incubator set at 39°C (102°F) for 5 to 10 minutes. Monitor the patient carefully…
The basic principles of fluid therapy are the same in the reptile as seen in birds and mammals, however reptile anatomy and physiology make some features of this crucial supportive care procedure unique. This article reviews fluid resuscitation with the use of crystalloid fluids and colloids, indications for replacement fluids including signs of dehydration and osmolarity values reported in reptiles. Routes of fluid administration in reptiles are described include subcutaneous, oral, soaking, intracoelomic, intraosseous, and intravenous via the cephalic vein, jugular vein, and in rare instances intracardiac catheter placement. Patient monitoring, including blood pressure measurement and signs of overhydration, are also explored.
Among exotic animals, venous cutdown is most commonly employed in reptiles like lizards and snakes although intraosseous catheters are also placed in lizards. Similarly, when an exotic companion mammal like a ferret or rabbit suffers from severe hypovolemia, dehydration, hypotension, and vascular collapse, intraosseous catheters have largely replaced venous cutdowns but this technique is occasionally employed.
Fluid therapy is an important part of supportive care, and there are several routes available for fluid support in the reptile. Subcutaneous and/or oral fluids are appropriate for mild to moderate dehydration, while intracoelomic, intravenous, or intraosseous fluids are administered to critically ill reptiles or to patients with moderate to severe dehydration.
All but the weakest ferrets can be challenging to restrain for blood collection. Consider sedation or general anesthesia, particularly if the handler or phlebotomist is relatively inexperienced; however remember that anesthesia can affect ferret hematology.
Use this video clip or article with still images to review equipment needed, and potential venipuncture sites including the jugular vein, cranial vena cava, lateral saphenous vein, and cephalic vein.
The rabbit has a relatively short prothrombin time and whole blood quickly clots at room temperature. To minimize the risk of clot formation, it can be helpful to pre-heparinize the needle and syringe by drawing heparin into the needle and expelling the excess from the hub. The total volume of blood that can be safely collected typically ranges from 0.5% to 1.0% body weight. Collect smaller volumes from geriatric patients or those suspected to have anemia or hypoproteinemia.
Use this video clip or article with still images to review equipment needed, and potential venipuncture sites including the jugular vein, lateral saphenous vein, and ear vessels.
Fluid therapy is a vital part of avian medicine, and appropriate administration of fluids is essential. Intravenous catheters are commonly used intraoperatively or in more stable hospitalized patients. Unfortunately intravenous catheter placement in birds can be challenging. The veins can be difficult to access and the vessels are also prone to hematoma formation.
Use this video clip or text with still images to review the equipment needed, the technique involved, and potential venipuncture sites including the jugular vein, medial metatarsal vein and basilic or ulnar vein.
Hematological and serum chemistry tests are considered part of the minimum database, yet collecting blood samples from small mammals can be extremely challenging. This review article reviews the recommended venipuncture site in popular exotic companion mammals including many rodents, rabbits, ferrets, hedgehogs, and sugar gliders. Sample collection from peripheral vessels including the cephalic, saphenous, tail, jugular, ear, and submandibular vein is discussed.
Blind venipuncture sites such as the cranial vena cava and femoral vessels are also described. Veterinary health professionals are also acquainted with the potential risks associated with blood collection from these small species, especially those presenting in advanced diseased states. Tips for clinical success are also shared.